pathogens

In the present study, biosynthesis of silver nanoparticles and its activity on water borne bacterialpathogens were investigated.

pomegranate

ABSTRACT This paper describes a simple and efficient procedure based on the green method for the preparation of silver nanoparticles with a relatively narrow distribution in size. Stable silver nanoparticles have been synthesized by using Pomegranate fruit extract as both the reducing and stabilizing agents. The morphology and size distribution of prepared silver nanoparticles varied with the concentration of the Pomegranate extract used. Nanoparticles thus prepared are found to be stable in aqueous solution over a period of three months at room temperature. The amount of silver nanoparticles synthesized and its qualitative characterization was done by UV-Vis absorption spectroscopy, FTIR, XRD, FESEM and EDAX analysis. The UV-Vis spectrum of silver nanoparticle in aqueous solution shows an absorbance peak around 420 nm due to Surface Plasmon Resonance. The XRD analysis revealed the fcc geometry of silver nanoparticles. The formation and stabilization of the nanoparticle is confirmed by FESEM analysis. The Silver nanoparticles displayed efficient antimicrobial activity towards most of the tested fungal and bacterial cultures. The cytotoxicity of the prepared silver nanoparticles was investigated using a cancer cell line.